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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two further splice variants had been noted but thorough nucleotide sequence information is not nonetheless available.
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Blocking peptides are peptides that bind specifically for the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded by the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This manual outlines even more feasible optimization of cellular and immunoassay parameters to be certain the very best outcomes are...
Antibodies sure to the blocking peptide no more bind to the epitope around the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinct expression and degradation patterns which add to the temporal coordination of each and every here mitotic function. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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